Herein, we aimed to evaluate homocysteine hcy as a biomarker for dr screening. Buy homocysteine elisa kit, human homocysteine elisa kit mbs7252797 product datasheet at. For concrete data please consult the instruction for use in the download box on the. Pdf a rapid and precise immunoassay for quantification of total homocysteine in blood samples is. Homocysteine elisa the axis homocysteine enzyme immunoassay is intended for the quantitative determination of total homocysteine in plasma or serum. Targetspecific elisa kits are available from a variety of manufacturers and can help streamline your immunodetection experiments. Effect of seabuckthorn seed oil in reducing cardiovascular risk factors. The kit has a detection sensitivity limit of 10 ngml homocysteine bsa.
The maxdiscovery homocysteine enzymatic assay kit has the capacity for 48 determinations or testing of 24 samples in duplicate assuming 12 wells for standards. This kit is not approved for use in humans or for clinical diagnosis. Homocysteine elisa kit okeh02557 quantitative competitive. Oct 12, 2017 ginsenoside rb1 prevents homocysteineinduced epc dysfunction via vegfp38mapk and sdf1cxcr4 activation. The sample and conjugate compete for antibody binding, which. Description and principle the elisa genie hcy elisa kit assay can assay for the specific analyte in the. Elisa kit for the detection of homocysteine in the research. Total homocysteine thcy plasma abbott imx homocysteine hcy. Hcyhomocysteine elisa kit from elisa genie is a precoated immunoassay with a sensitivity of. Introduction over 1 million of americans died of cardiovascular disease cvd such as heart attack or stoke.
A longitudinal controlled trial on hypertensive subjects. Since vitamins b6, b12, and folate are necessary to metabolize homocysteine, increased levels of the amino acid may be a sign of deficiency in those vitamins. Homocysteine test kit homocysteine control kit homocysteine calibrator kit kit size 16 testspack 2 x 1 ml high and low 1 x 1 ml summary homocysteine is a thiol containingaminoacid produced by the intracellular demethylation of methionine. Bioassay technology laboratory bt lab is a brand of shanghai korain biotech co ltd specialized in lab supplies of elisa kit, antibodies, protein for life science research. Serum folic acid, vitamin b12, homocysteine and serotonin was done by elisa.
Homocysteine is an amino acid intermediate that is synthesized from methionine via sadenosylmethionine sam and sadenosylhomocysteine sah. Use the product attributes below to configure the comparison table. Homocysteine is a thiolcontaining amino acid produced by the intracellular demethylation of methionine. To avoid evaporation of the reagents, the reagent pack kit should not be kept open in the abbott imx analyzer when a run is finished. A number of b vitamins in particular folic acid, pyridoxal, and vitamin b12 are involved in homocysteine metabolism so that deficiencies of these vitamins are among the most common causes of. It is developed for quantitative measurement of homocysteine in rat serum, plasma and other biological fluids. Creative diagnostics provides homocysteine antibodies and elisa kits for science research use, feel free to contact us. Folic acid decreases astrocyte apoptosis by preventing oxidative stressinduced telomere attrition li w,international journal of molecular sciences,2019. The extent to which these factors affect blood hcy levels is shown in figures 2 and 3. It is a competitive eia assay which can detect homocysteine down to 98. Nbutyryllhomocysteine thiolactone is an analog of nbutyryllhomoserine lactone, the small diffusible signaling molecule involved in quorum sensing, thereby controlling gene expression and cellular metabolism. Diazyme receives 510k fda approval for homocysteine assay kit san diego, ca sept.
The antibody shop, the marketplace with over a million primary and secondary antibodies, elisa kits, qpcr kits, ria kits, assay kits and reagents. Homocysteine elisa kit deia3576 creative diagnostics. It is a homologue of the amino acid cysteine, differing by an additional methylene bridge ch2. The axis homocysteine enzyme immunoassay is intended for the. Standards preparation standards corresponding to 0. Ginsenoside rb1 prevents homocysteineinduced epc dysfunction. Caymans dna methylation elisa kit is a competitive assay that can be used for the quantification of 5methyl2deoxycytidine in urine, culture supernatants, plasma, and other sample matrices. We found significantly elevated levels of homocysteine in women with ppd as compared to those without ppd, both at 2448 h as well as six weeks after delivery, although no associations were found with folate and vitamin b12 levels. Elisa kit for the detection of homocysteine in the research laboratory the antibodyshop, antibodies, elisa kits, reagents. High homocysteine levels prevent via h2 s the cocl2 induced alteration of lymphocyte viability.
Raised levels of homocysteine have been known to increase the risk of developing heart disease, dementia and infertility. Linearity lq homocysteine, calibration verification. Maxdiscovery homocysteine enzymatic assay kit bioo scientific. The assay is based on the reduction of homocysteine disulfides to free homocysteine, which is cleaved by a homocysteine selective enzyme, generating an intermediate product. Homocysteine assay kit fluorometric ab228559 allows for quantification of total homocysteine in biological fluids such as plasma and serum. The eagle biosciences sadenosyllhomocysteine sah elisa assay kit is intended for the quantitative determination of sadenosyllhomocysteine sah in serum or plasma. Usually nm is nmoll,but most literatures use nmolmlsome literatures use nm, and it is due to habit. This assay is a sandwich enzyme linkedimmunosorbent assay elisa. Siemens immulite homocysteine 2000 user manual pdf download. To meet every single need of the customer we aim to provide high quality at best price and service with our professional staff. The yorktest homocysteine test is a quick and easy hometolab fingerprick blood test that is analysed in our laboratory within just 10 working days. Homocysteine elisa kit alpha diagnostic international. The eagle biosciences sadenosyllhomocysteine sah elisa assay kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.
This immunoassay kit allows for the in vitro quantitative determination of general homocysteine concentrations in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Evaluation of nitric oxide and homocysteine levels in primary. Homocysteine elisa kit lsf25097 is a 96well enzymelinked immunosorbent assay for the quantitative detection of all species homocysteine in plasma, serum. Identical methods and protocols were used for both groups. Aviva systems biology homocysteine kit human okeh02557 is based on standard sandwich enzymelinked immunosorbent assay technology. Homocysteine is an amino acid that is typically present in very small amounts in all cells of the body. Elisa kit for homocysteine hcy, competition elisa, cloud. The presence of elevated total homocysteine levels in plasma or serum has been connected to the increased risk of thrombosis, neural tube defects and atherosclerotic vascular disease. Homocysteine linearity is assayed calibration verification material consisting of five levels of human based serum. This elisa kit is used for quantitative measurement of homocysteine in serum, plasma, tissue homogenates and other biological fluids. Elisa kit suppliers from china shanghai korain biotech co. Lsf10591 it is important that you read this entire manual carefully before starting your experiment. For the quantitative determination of endogenic human homocysteine hcy concentrations in.
Pdf plasma homocysteine levels in multiple sclerosis. The kit has a detection sensitivity limit of 10 ngml homocysteinebsa. Nitric oxide assays were carried out using a specific enzymelinked immunosorbent assay elisa kit nitrate test kit, catalog mntk302 and homocysteine assays using a specific elisa kit for humans as described in the manufacturers manual. Total homocysteine biochemical assay kit intented use the formosa biomedical technology total homocysteine biochemical assay kit is an in vitro assay for the quantitative determination of total homocysteine in plasma. Maxdiscovery homocysteine enzymatic assay kit specs. Homocysteine test expert laboratory testing and analysis. Principle of the assay the microtiter plate provided in this kit has been precoated with an antibody specific to homocysteine. Elevated levels of homocysteine can indicate a risk for numerous diseases, including vascular and heart disease. Human homocysteine elisa kit allows for the in vitro quantitative determination of homocysteine concentrations in serum, plasma, tissue homogenates and cell culture supernates and other biological fluids.
That is because the body normally converts homocysteine into other products quickly. All species homocysteine elisa kit sandwich elisa user manual catalog no. The shelf life is 12 months when the kit is properly stored. Test principle the microtiter plate provided in human homocysteine elisa kit has been precoated with an antibody specific to homocysteine. Homocysteine elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Ced984ge, elisa kit for homocysteine hcy, panspecies general, competition elisa, designed by cloudclone corp. Homocystein elisa,homocystein,stroke, heart attack, homocystein elisa. Hcy elisa kit human hcy homocysteine elisa kit mybiosource. Diazyme receives 510k fda approval for homocysteine assay kit. This assay is a competitive elisa where samples and an anti homocysteine antibody are added to a plate coated with a homocysteine conjugate. Immulite homocysteine 2000 medical equipment pdf manual download. Correlation of tsh with homocysteine and lipid profile in hypothyroidism a malik, et al,j liaquat uni med health sci,2018. Pdf enzyme conversion immunoassay for determining total.
T here are several factors that cause elevation of hcy levels in blood. A competitive elisa kit for the quantitative measurement of homocysteine in serum, plasma, culture supernatants and other biological fluids. Cell biolabs homocysteine elisa kit is a competitive enzyme immunoassay developed for the detection and quantitation of homocysteine in plasma, serum, lysates, or other biological fluid samples. Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats mustafa ahmet huyut, et al,international journal of the cardiovascular academy,2019. Correlation of serum lipids, lipoproteins and homocysteine levels in hypothyroid patients of northwest india kuldip singh, et al,international journal of scientific research,2018. This kit csbe16551m is specific to detect homocysteine. Our homocysteine elisa is designed for detection and quantitation of homocysteine in plasma, serum, lysates, or biological fluid samples.
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